Characterization of the replication cycle of the Lymantria dispar nuclear polyhedrosis virus
نویسندگان
چکیده
منابع مشابه
Characterization of the replication cycle of the Lymantria dispar nuclear polyhedrosis virus.
The life cycle of the Lymantria dispar nuclear polyhedrosis virus (LdMNPV) was characterized through analysis of budded virus (BV) release, the temporal formation of polyhedra, the temporal transcription pattern of representative early, late, and hyper-expressed late genes, and the onset of DNA replication in the Ld652Y cell line. Transcripts from the LdMNPV immediate early gene G22 were detect...
متن کاملCharacterization of Gypsy Moth (Lymantria dispar) Nuclear Polyhedrosis Virus.
Characterization of the proteins and nucleic acid of the gypsy moth nuclear polyhedrosis virus isolated in Ithaca, N.Y. (LdNPV-IT) is presented. A total of 29 viral structural proteins were identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis when the virus was isolated in the absence of alkaline protease activity. Fourteen surface envelope viral proteins were identified by l...
متن کاملCharacterization of the nucleotide sequence of the Lymantria dispar nuclear polyhedrosis virus DNA polymerase gene region.
The DNA polymerase gene of the Lymantria dispar multinucleocapsid nuclear polyhedrosis virus (LdMNPV) was cloned and sequenced. The predicted DNA polymerase protein (1113 amino acids, 115.9K) was found to have an amino acid identity of 48% with the corresponding gene of the Autographa californica MNPV (AcMNPV). It contains five domains associated with substrate binding, primase interaction, and...
متن کاملIdentification and characterization of an early gene in the Lymantria dispar multinucleocapsid nuclear polyhedrosis virus.
The Lymantria dispar multinucleocapsid nuclear polyhedrosis virus (LdMNPV) gene encoding G22 was cloned and sequenced. The G22 gene codes for a 191 amino acid protein with a predicted M(r) of 22,000. Expression of G22 in a rabbit reticulocyte system generated a protein with an M(r) of 24,000, in close agreement with the molecular mass predicted from the nucleotide sequence. G22 is not significa...
متن کاملIdentification and characterization of a protein kinase gene in the Lymantria dispar multinucleocapsid nuclear polyhedrosis virus.
The Lymantria dispar multinucleocapsid nuclear polyhedrosis virus (LdMNPV) gene encoding vPK has been cloned and sequenced. LdMNPV vPK shows a 24% amino acid identity to the catalytic domains of the eucaryotic protein kinases nPKC from rabbits, HSPKCE from humans, APLPKCB from Aplysia californica, and dPKC98F from Drosophila melanogaster, and homology to several other protein kinases from yeast...
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ژورنال
عنوان ژورنال: Virus Research
سال: 1997
ISSN: 0168-1702
DOI: 10.1016/s0168-1702(97)00075-0